Coding
Part:BBa_K3930026:Design
Designed by: Thomas Gaudin Group: iGEM21_Toulouse_INSA-UPS (2021-10-08)
3,6-nonadienal induction system and expression in S. elongatus (pCONCOMBRE)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2244
Illegal EcoRI site found at 2250
Illegal XbaI site found at 590
Illegal XbaI site found at 6286
Illegal XbaI site found at 8003
Illegal XbaI site found at 8030
Illegal PstI site found at 8476 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2244
Illegal EcoRI site found at 2250
Illegal NheI site found at 777
Illegal NheI site found at 872
Illegal NheI site found at 899
Illegal PstI site found at 8476
Illegal NotI site found at 7951
Illegal NotI site found at 7959 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2244
Illegal EcoRI site found at 2250
Illegal BglII site found at 6283
Illegal BglII site found at 6289
Illegal BamHI site found at 3717
Illegal XhoI site found at 3753
Illegal XhoI site found at 4736
Illegal XhoI site found at 6361 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2244
Illegal EcoRI site found at 2250
Illegal XbaI site found at 590
Illegal XbaI site found at 6286
Illegal XbaI site found at 8003
Illegal XbaI site found at 8030
Illegal PstI site found at 8476 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2244
Illegal EcoRI site found at 2250
Illegal XbaI site found at 590
Illegal XbaI site found at 6286
Illegal XbaI site found at 8003
Illegal XbaI site found at 8030
Illegal PstI site found at 8476
Illegal NgoMIV site found at 1936
Illegal AgeI site found at 2163
Illegal AgeI site found at 2190
Illegal AgeI site found at 4955
Illegal AgeI site found at 7844 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 8686
Illegal BsaI.rc site found at 911
Illegal SapI site found at 2653
Illegal SapI site found at 3728
Illegal SapI.rc site found at 7355